CD33 Monoclonal antibody

CD33 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B2E5

Cat no : 67135-1-Ig

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

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Validation Data Gallery

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  WB analysis of THP-1 using 67135-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67135-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 67135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 67135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 67135-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (67135-1-Ig, Clone: 1B2E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67135-1-Ig targets CD33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD33 fusion protein Ag11402
• Full Name: CD33 molecule
• Calculated Molecular Weight: 364 aa, 40 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC028152
• Gene Symbol: CD33
• Gene ID (NCBI): 945
• RRID: AB_2882434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

Publications

Species	Application	Title
human	IF	Front Med (Lausanne); Prognostic significance of peripheral and tumor-infiltrating lymphocytes in newly diagnosed stage III/IV non-small-cell lung cancer; Authors - Fenge Li