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Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody, PBS Only

CD324 (E-cadherin) Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240159G8

Cat no : 98030-1-PBS

Synonyms

E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using Anti-Human CD324 (E-cadherin) Rabbit Recombinant A (98030-1-RR)

    FC experiment of MCF-7 using 98030-1-RR (same clone as 98030-1-PBS)

    1x10^6 MCF-7 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone: 240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98030-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using Anti-Human CD324 (E-cadherin) Rabbit Recombinant A (98030-1-RR)

    FC experiment of HT-29 using 98030-1-RR (same clone as 98030-1-PBS)

    1x10^6 HT-29 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98030-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98030-1-RR

    Affinity and Kinetic Characterization of 98030-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98030-1-RR against Human E-cadherin were performed. The affinity constant is 37.3 pM.

Product Information

The immunogen of 98030-1-PBS is CD324 (E-cadherin) Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD324 (E-cadherin) fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight 882 aa, 97 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.