Anti-Human CD314/NKG2D (1D11) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65643-1-MR (same clone as 65643-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD314/NKG2D (1D11) Mouse IgG2a Recombinant Antibody (65643-1-MR, Clone:1D11) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65643-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65643-1-MR (same clone as 65643-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD314/NKG2D (1D11) Mouse IgG2a Recombinant Antibody (65643-1-MR, Clone:1D11) (red) or Mouse IgG2a Isotype Control (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65643-1-PBS in a different storage buffer formulation.

CD314/NKG2D Recombinant Antibody for FC

Cat No. 65643-1-PBS

Clone No.1D11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

KLRK1, CD314, 1D11, KLR, Killer cell lectin-like receptor subfamily K member 1

---

Product Information

65643-1-PBS targets CD314/NKG2D in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: NKL cell line
• Full Name: killer cell lectin-like receptor subfamily K, member 1
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC039836
• Gene Symbol: KLRK1
• Gene ID (NCBI): 22914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).