FITC Plus Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (2)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

Publications

4

CloneNo.

390

Cat no : FITC-65058

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Validation Data Gallery

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  IF Staining of bEnd.3 using FITC-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100.

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  FC experiment of C57BL/6 mouse bone marrow cells using FITC-65058

  1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD45R (B220) and 0.5 ug FITC Plus Anti-Mouse CD31 (FITC-65058, Clone: 390) or 0.5 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were not fixed.

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Tested Applications

• Positive IF detected in: bEnd.3 cells
• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 3 publications below

Product Information

FITC-65058 targets CD31 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: Pecam1
• Gene ID (NCBI): 18613
• RRID: AB_2883748
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
mouse	IF	Cell Death Dis; LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease.; Authors - Hong Su
mouse	FC	Bone; mTORC1 coordinates NF-κB signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification; Authors - Jiaming Fu
rat	FC	Cells; Enhancement of Tendon Repair Using Tendon-Derived Stem Cells in Small Intestinal Submucosa via M2 Macrophage Polarization; Authors - Xufeng Mao
mouse	FC	Biochim Biophys Acta Mol Cell Res; CARM1 deficiency inhibits osteoblastic differentiation of bone marrow mesenchymal stem cells and delays osteogenesis in mice; Authors - Jing-Yi Li