Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC

Conjugate

Unconjugated

Publications

8

CloneNo.

390

Cat no : 65058-1-Ig

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Validation Data Gallery

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  IF Staining of bEnd.3 using 65058-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (65058-1-Ig, Clone: 390 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of mouse splenocytes using 65058-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD31 (65058-1-Ig, Clone: 390) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: bEnd.3 cells
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 7 publications below

Product Information

65058-1-Ig targets CD31 in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• RRID: AB_2918366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
	IF	mBio; Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malaria; Authors - Rui Liang
mouse	IF	J Ethnopharmacol; Investigation of the efficacy of Dengzhan Shengmai capsule against heart failure with preserved ejection fraction; Authors - Ziyi Kang
mouse	IF	J Ethnopharmacol; Berberis dictyophylla F. inhibits angiogenesis and apoptosis of diabetic retinopathy via suppressing HIF-1α/VEGF/DLL-4/Notch-1 pathway.; Authors - Xiaopeng Ai
mouse	IF	Eur J Pharmacol; Crosstalk between endothelial cells with a non-canonical EndoMT phenotype and cardiomyocytes/fibroblasts via IGFBP5 aggravates TAC-induced cardiac dysfunction; Authors - Yue Li
		Phytother Res; A new look at angiogenesis inhibition of geniposide in experimental arthritis by blocking angiopoietin-2 exocytosis; Authors - Peirong Gan
mouse	IF	Adv Mater; Photothermal and Photocatalytic Glycol Chitosan and Polydopamine-Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO1-x I) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds; Authors - Jianglong Kong