CD300a Recombinant antibody

CD300a Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242131H5

Cat no : 84674-4-RR

Synonyms

CMRF 35 H9, CLM-8, CLM 8, CD300a molecule, CD300 antigen-like family member A

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Validation Data Gallery

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  WB analysis using 84674-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84674-4-RR (CD300A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 84674-4-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD300A antibody (84674-4-RR, Clone: 242131H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84674-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84674-4-RR against Human CD300A were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84674-4-RR targets CD300a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD300a molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 40-70 kDa
• GenBank Accession Number: NM_007261.4
• Gene Symbol: CD300A
• Gene ID (NCBI): 11314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human CD300a is a transmembrane protein, with an immunoglobulin (Ig)V-like extracellular domain and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs), providing the receptor with an inhibitory capacity. The relevance of the CD300a molecule in several pathological conditions has been highlighted by multiple studies. (PMID: 37762055)