CD30/TNFRSF8 Recombinant monoclonal antibody

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  WB analysis of Karpas-299 using 84241-2-RR

  Karpas-299 cells were subjected to SDS PAGE followed by western blot with 84241-2-RR (CD30/TNFRSF8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84241-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84241-2-RR against Human CD30/TNFRSF8 were performed. The affinity constant is 0.391 nM.

CD30/TNFRSF8 Recombinant Antibody for WB, ELISA

Cat No. 84241-2-RR

Clone No.241330G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD30, 241330G11, Ki 1 antigen, Ki 1, D1S166E

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Tested Applications

• Positive WB detected in: Karpas-299 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84241-2-RR targets CD30/TNFRSF8 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_001243
• Gene Symbol: CD30
• Gene ID (NCBI): 943
• RRID: AB_3671793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).