Anti-Human CD30/TNFRSF8 Rabbit Recombinant Antibody, PBS Only

CD30/TNFRSF8 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241330A12

Cat no : 98124-1-PBS

Synonyms

CD30, 241330A12, Ki 1 antigen, Ki 1, D1S166E

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Validation Data Gallery

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  FC experiment of human PBMCs using 98124-1-RR (same clone as 98124-1-PBS)

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR, Clone: 241330A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), 0.25 ug or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR, Clone: 241330A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98124-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98124-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98124-1-RR against Human CD30 were performed. The affinity constant is 0.528 nM.

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Product Information

98124-1-PBS targets CD30/TNFRSF8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 64 kDa
• GenBank Accession Number: NM_001243
• Gene Symbol: CD30
• Gene ID (NCBI): 943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).