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CD3 Gamma Recombinant antibody, PBS Only

CD3 Gamma Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241849E4

Cat no : 84836-1-PBS

Synonyms

CD3G, 241849E4, T-cell receptor T3 gamma chain, T3G, T cell receptor T3 gamma chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 Gamma Recombinant antibody (84836-1-RR)

    WB analysis using 84836-1-RR (same clone as 84836-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84836-1-RR (CD3 Gamma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Gamma Recombinant antibody (84836-1-RR)

    IHC staining of human tonsillitis using 84836-1-RR (same clone as 84836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84836-1-RR (CD3 Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84836-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Gamma Recombinant antibody (84836-1-RR)

    IHC staining of human tonsillitis using 84836-1-RR (same clone as 84836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84836-1-RR (CD3 Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84836-1-PBS in a different storage buffer formulation.

Product Information

84836-1-PBS targets CD3 Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD3g molecule, gamma (CD3-TCR complex)
Calculated Molecular Weight 182 aa, 20 kDa
Observed Molecular Weight20-25 kDa
GenBank Accession NumberBC113830
Gene Symbol CD3 Gamma
Gene ID (NCBI) 917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.