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CD3 Delta Monoclonal antibody, PBS Only (Capture)

CD3 Delta Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12H2

Cat no : 60194-1-PBS

Synonyms

CD3D, 3A12H2, T-cell receptor T3 delta chain, T3D, T cell receptor T3 delta chain

Validation Data Gallery

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  • Western Blot (WB) analysis of jurkat cells using CD3 Delta Monoclonal antibody (60194-1-Ig)

    WB analysis of jurkat using 60194-1-Ig (same clone as 60194-1-PBS)

    jurkat cells were subjected to SDS PAGE followed by western blot with 60194-1-Ig (CD3 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60194-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50691-2

    Cytometric bead array standard curve of MP50691-2

    Cytometric bead array standard curve of MP50691-2, CD3D/CD3 Delta Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60194-1-PBS. Detection antibody: 60194-4-PBS. Standard:Ag10207. Range: 0.781-100 ng/mL.

Product Information

60194-1-PBS targets CD3 Delta as part of a matched antibody pair:

MP50691-2: 60194-1-PBS capture and 60194-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD3 Delta fusion protein Ag10207
Full Name CD3d molecule, delta (CD3-TCR complex)
Calculated Molecular Weight 171 aa, 19 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC070321
Gene Symbol CD3 Delta
Gene ID (NCBI) 915
RRIDAB_10898175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. CD3 delta/CD3D is a single-pass type I membrane protein which consists of an extracellular domain of 84 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. Defects in CD3 delta are a cause of severe combined immunodeficiency.