CD3 Delta Polyclonal antibody

CD3 Delta Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 32144-1-AP

Synonyms

CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, T3D, T cell receptor T3 delta chain

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Validation Data Gallery

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  WB analysis using 32144-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32144-1-AP (CD3 Delta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32144-1-AP targets CD3 Delta in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD3d molecule, delta (CD3-TCR complex)
• Calculated Molecular Weight: 171 aa, 19 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC070321
• Gene Symbol: CD3 Delta
• Gene ID (NCBI): 915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.