FITC Plus Anti-Human CD3 (OKT3) Rabbit Recombinant Antibody

CD3 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

OKT3

Cat no : FITC-65504

Synonyms

CD3E

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65504

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD45 and 5 ul FITC Plus Anti-Human CD3 Rabbit Recombinant Antibody (FITC-65504, Clone:OKT3) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65504

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 Rabbit Recombinant Antibody (FITC-65504, Clone:OKT3) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65504

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD45 and 5 ul FITC Plus Anti-Human CD3 Rabbit Recombinant Antibody (FITC-65504, Clone:OKT3) (left) or 5 ul FITC Plus Anti-Human CD3 Mouse Monoclonal Antibody (FITC-65133, Clone:OKT3) (right). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65504 targets CD3 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T cells
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).