Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody, PBS Only

CD3 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT1

Cat no : 65570-1-PBS

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

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Validation Data Gallery

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  FC experiment of human PBMCs using 65570-1-MR (same clone as 65570-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 Mouse IgG2a Recombinant Antibody (65570-1-MR, Clone: UCHT1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65570-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65570-1-PBS

  1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody, PBS Only (65570-1-PBS, Clone:UCHT1) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65570-1-MR (same clone as 65570-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (65570-1-MR, Clone: UCHT1) or 0.25 ug 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 65570-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65570-1-PBS

  1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody, PBS Only (65570-1-PBS, Clone:UCHT1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (right), or 0.5 ug Anti-Human CD3 (UCHT1) (65151-1-Ig, Clone: UCHT1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (left). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65570-1-PBS targets CD3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).