Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody, PBS Only

CD3 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT3

Cat no : 65569-1-PBS

Synonyms

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

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Validation Data Gallery

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  FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19, and 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone:OKT3) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65569-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone: OKT3) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65569-1-PBS in a different storage buffer formulation.

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Product Information

65569-1-PBS targets CD3 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T cells
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).