Anti-Human CD29 (TS2/16) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65601-1-MR (same clone as 65601-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (65601-1-MR, Clone: TS2/16) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD45. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65601-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65601-1-MR (same clone as 65601-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (65601-1-MR, Clone: TS2/16) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65601-1-PBS in a different storage buffer formulation.

CD29 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65601-1-PBS

Clone No.TS2/16

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA

---

Product Information

65601-1-PBS targets CD29 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC020057
• Gene Symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• ENSEMBL Gene ID: ENSG00000150093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.