Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody, PBS Only

CD28 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

CD28.2

Cat no : 65621-1-PBS

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28.2, CD28 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody (65621-1-MR, Clone: CD28.2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65621-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody (65621-1-MR, Clone: CD28.2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65621-1-PBS in a different storage buffer formulation.

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Product Information

65621-1-PBS targets CD28 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD28 molecule
• Calculated Molecular Weight: 220 aa, 25 kDa
• GenBank Accession Number: BC093698
• Gene Symbol: CD28
• Gene ID (NCBI): 940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).