B7-H3/CD276 Recombinant antibody

B7-H3/CD276 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230305A10

Cat no : 83152-5-RR

Synonyms

4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

---

Validation Data Gallery

• 

  WB analysis using 83152-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83152-5-RR (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83152-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83152-5-RR against Human CD276 were performed. The affinity constant is 0.607 nM.

---

Tested Applications

• Positive WB detected in: U2OS cells, HEK-293 cells, HeLa cells, LNCaP cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83152-5-RR is B7-H3/CD276 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: NM_001024736
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).