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B7-H3 Polyclonal antibody

B7-H3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14453-1-AP

Synonyms

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352



Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, U2OS cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue, human medulloblastoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14453-1-AP targets B7-H3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene symbol CD276
Gene ID (NCBI) 80381
RRIDAB_2073577
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
WB protocol for B7-H3 antibody 14453-1-APDownload protocol
IHC protocol for B7-H3 antibody 14453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Nutr Biochem

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Authors - Qi Zhang
humanWB

J Cell Physiol

Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.

Authors - Lipeng Tang
humanIHC

CNS Neurosci Ther

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

Authors - Hao Zhang
humanIHC

J Cancer

The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.

Authors - Zhangao Li
humanIHC

Med Oncol

Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.

Authors - Miao Li
humanWB

J Oncol

Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.

Authors - Qiu-Ting Li