Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, LNCaP cells, U2OS cells
|Positive IHC detected in
|human prostate cancer tissue, human testis tissue, human medulloblastoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:8000
|IHC : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14453-1-AP targets B7-H3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|B7-H3 fusion protein Ag6006
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
J Nutr Biochem
Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression
J Cell Physiol
Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.
CNS Neurosci Ther
PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.
The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.
Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.
Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.