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B7-H3/CD276 Polyclonal antibody

B7-H3/CD276 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 14453-1-AP


4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, U2OS cells
Positive IHC detected inhuman prostate cancer tissue, human testis tissue, human medulloblastoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14453-1-AP targets B7-H3/CD276 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B7-H3/CD276 fusion protein Ag6006
Full Name CD276 molecule
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC062581
Gene Symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).


Product Specific Protocols
WB protocol for B7-H3/CD276 antibody 14453-1-APDownload protocol
IHC protocol for B7-H3/CD276 antibody 14453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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