Mouse CD27 Recombinant antibody, PBS Only (Capture/Detector)

CD27 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241378E11

Cat no : 84157-4-PBS

Synonyms

Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01069-2

  Sandwich ELISA standard curve of MP01069-2, Mouse CD27 Recombinant Matched Antibody Pair - PBS only. 84157-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1311. 84157-4-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

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  Sample test of MP01069-2

  The mean CD27 concentration was determined to be 89.6 ng/mL in mouse thymus cell extract based on a 1.3 mg/mL extract load and 22.2 ng/mL in mouse spleen cell extract based on a 2.9 mg/mL extract load.

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  Cytometric bead array standard curve of MP01069-1

  Cytometric bead array standard curve of MP01069-1, MOUSE CD27/TNFRSF7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84157-4-PBS. Detection antibody: 84157-3-PBS. Standard: Eg1311. Range: 0.781-25 ng/mL.

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  Cytometric bead array standard curve of MP01069-2

  Cytometric bead array standard curve of MP01069-2, MOUSE CD27/TNFRSF7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84157-2-PBS. Detection antibody: 84157-4-PBS. Standard: Eg1311. Range: 0.781-50 ng/mL.

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  Cytometric bead array standard curve of MP01069-3

  Cytometric bead array standard curve of MP01069-3, MOUSE CD27/TNFRSF7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84157-1-PBS. Detection antibody: 84157-4-PBS. Standard: Eg1311. Range: 0.781-50 ng/mL.

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Product Information

84157-4-PBS targets CD27 as part of a matched antibody pair:

MP01069-1: 84157-4-PBS capture and 84157-3-PBS detection (validated in Cytometric bead array)

MP01069-2: 84157-2-PBS capture and 84157-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01069-3: 84157-1-PBS capture and 84157-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD27 antigen
• Calculated Molecular Weight: 28kDa
• GenBank Accession Number: NM_001033126.2
• Gene Symbol: CD27
• Gene ID (NCBI): 21940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.