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Mouse CD27 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-50 ng/mL

Sandwich ELISA: 9.77-625 pg/mL

Cat No : MP01069-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01069-2, Mouse CD27 Recombinant Matched Antibody Pair - PBS only. 84157-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1311. 84157-4-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

    Sandwich ELISA standard curve of MP01069-2

    Sandwich ELISA standard curve of MP01069-2, Mouse CD27 Recombinant Matched Antibody Pair - PBS only. 84157-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1311. 84157-4-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

  • The mean CD27 concentration was determined to be 89.6 ng/mL in mouse thymus cell extract based on a 1.3 mg/mL extract load and 22.2 ng/mL in mouse spleen cell extract based on a 2.9 mg/mL extract load.

    Sample test of MP01069-2

    The mean CD27 concentration was determined to be 89.6 ng/mL in mouse thymus cell extract based on a 1.3 mg/mL extract load and 22.2 ng/mL in mouse spleen cell extract based on a 2.9 mg/mL extract load.

  • Cytometric bead array standard curve of MP01069-2, MOUSE CD27/TNFRSF7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84157-2-PBS. Detection antibody: 84157-4-PBS. Standard: Eg1311. Range: 0.781-50 ng/mL.

    Cytometric bead array standard curve of MP01069-2

    Cytometric bead array standard curve of MP01069-2, MOUSE CD27/TNFRSF7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84157-2-PBS. Detection antibody: 84157-4-PBS. Standard: Eg1311. Range: 0.781-50 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01069-2 targets CD27 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-50 ng/mL (Cytometric Bead Array); 9.77-625 pg/mL (Sandwich ELISA);

Capture antibody: Mouse CD27 Recombinant antibody, PBS Only (Capture) 84157-2-PBS (241378C1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse CD27 Recombinant antibody, PBS Only (Capture/Detector) 84157-4-PBS (241378E11). 100 μg. Concentration 1 mgl/ml.

Alternative CD27 matched antibody pairs: MP01069-1, MP01069-3

Matched pair protein standard: Eg1311

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84157-2-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD27 antigen
Synonyms Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen
GenBank accession numberNM_001033126.2
Gene symbol CD27
UniprotP41272
Gene ID (NCBI) 21940
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84157-4-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD27 antigen
Synonyms Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen
GenBank accession numberNM_001033126.2
Gene symbol CD27
UniprotP41272
Gene ID (NCBI) 21940
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.