Anti-Mouse CD27 Rabbit Recombinant Antibody, PBS Only

CD27 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241378E11

Cat no : 98160-1-PBS

Synonyms

Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98160-1-RR (same clone as 98160-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR, Clone:241378E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). cells were co-stained with APC Anti-mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98160-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98160-1-RR (same clone as 98160-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR, Clone: 241378E11) (red), or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98160-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98160-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98160-1-RR against Mouse CD27 were performed. The affinity constant is 1.87 nM.

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Product Information

The immunogen of 98160-1-PBS is CD27 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD27 antigen
• Calculated Molecular Weight: 28kDa
• GenBank Accession Number: NM_001033126.2
• Gene Symbol: Cd27
• Gene ID (NCBI): 21940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.