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Anti-Mouse CD27 Rabbit Recombinant Antibody

CD27 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241378E11

Cat no : 98160-1-RR

Synonyms

Tumor necrosis factor receptor superfamily member 7, Tnfrsf7, T-cell activation antigen CD27, CD27L receptor, CD27 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR)

    FC experiment of mouse splenocytes using 98160-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR, Clone:241378E11) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). cells were co-stained with APC Anti-mouse CD3. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR)

    FC experiment of mouse splenocytes using 98160-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD27 Rabbit Recombinant Antibody (98160-1-RR, Clone: 241378E11) (red), or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98160-1-RR

    Affinity and Kinetic Characterization of 98160-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98160-1-RR against Mouse CD27 were performed. The affinity constant is 1.87 nM.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98160-1-RR is CD27 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD27 antigen
Calculated Molecular Weight28kDa
GenBank Accession NumberNM_001033126.2
Gene Symbol Cd27
Gene ID (NCBI) 21940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocols

Product Specific Protocols
FC protocol for CD27 antibody 98160-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols