Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody

CD26 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BA5b

Cat no : 65574-1-MR

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Validation Data Gallery

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  FC experiment of human PBMCs using 65574-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD26 Mouse IgG2a Recombinant Antibody (65574-1-MR, Clone: BA5b) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65574-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD26 Mouse IgG2a Recombinant Antibody (65574-1-MR, Clone: BA5b) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65574-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD26 Mouse IgG2a Recombinant Antibody (65574-1-MR, Clone: BA5b) (left) or 0.25 ug Anti-Human CD26 (65267-1-Ig, Clone: BA5b) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65574-1-MR targets CD26 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: A human T cell clone
• Full Name: dipeptidyl-peptidase 4
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC013329
• Gene Symbol: DPP4
• Gene ID (NCBI): 1803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).