Anti-Human CD244 Rabbit Recombinant Antibody, PBS Only

CD244 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241260G10

Cat no : 98133-1-PBS

Synonyms

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

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Validation Data Gallery

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  FC experiment of human PBMCs using 98133-1-RR (same clone as 98133-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR, Clone: 241260G10) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98133-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98133-1-RR (same clone as 98133-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD244 Rabbit Recombinant Antibody (98133-1-RR, Clone:241260G10) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98133-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98133-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98133-1-RR against Human CD244 were performed. The affinity constant is 1.29 nM.

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Product Information

The immunogen of 98133-1-PBS is CD244 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD244 molecule, natural killer cell receptor 2B4
• Calculated Molecular Weight: 365 aa, 41 kDa
• GenBank Accession Number: BC053985
• Gene Symbol: CD244
• Gene ID (NCBI): 51744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).