FITC Plus Anti-Human Glycophorin A/CD235a (HIR2)

• 

  FC experiment of human red blood cells using FITC-65174

  1X10^6 human red blood cells were surface stained with 5 ul FITC Anti-Human CD235a (FITC-65174, Clone: HIR2) (red) or Mouse IgG2b Isotype Control. Cells were not fixed.

Glycophorin A/CD235a Monoclonal Antibody for FC

Cat No. FITC-65174

Clone No.HIR2

Publications(1)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Synonyms

Glycophorin A, CD235a, GpMiIII, GPErik, GPA

---

Tested Applications

• Positive FC detected in: human red blood cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65174 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Synthetic peptide (Human, N-terminal)
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• RRID: AB_2883814
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P02724
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Publications

Species	Application	Title
		Int J Nanomedicine; Non-Invasive Prenatal Diagnosis of Chromosomal and Monogenic Disease by a Novel Bioinspired Micro-Nanochip for Isolating Fetal Nucleated Red Blood Cells; Authors - Naiqi Li