Mouse CD23/Fc epsilon RII Recombinant antibody, PBS Only (Detector)

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  WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01134-2

  Sandwich ELISA standard curve of MP01134-2, Mouse CD23 Recombinant Matched Antibody Pair - PBS only. 84212-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1295. 84212-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01134-2

  Serum of six mouse was measured. The CD23 concentration of detected samples was determined to be 2624.00 pg/mL with a range of 1279.00 - 7445.00 pg/mL.

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  Affinity and Kinetic Characterization of 84212-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

CD23/Fc epsilon RII Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84212-3-PBS

Clone No.241385C9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241385C9

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Product Information

84212-3-PBS targets CD23/Fc epsilon RII as part of a matched antibody pair:

MP01134-2: 84212-4-PBS capture and 84212-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Fc receptor, IgE, low affinity II, alpha polypeptide
• Calculated Molecular Weight: 38kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: EDL21948.1
• Gene Symbol: Fcer2a
• Gene ID (NCBI): 14128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20693-1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.