1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98002-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98002-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000; or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:11A8) (center) or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:DX11) (right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000; or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:11A8) (center) or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:DX11) (right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
98002-1-PBS targets CD226 in FC applications and shows reactivity with human samples.
Store at -80°C. Stable for one year after shipment.
Background Information
CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98002-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98002-1-PBS
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-PBS, Clone:41A3) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000; or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:11A8) (center) or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:DX11) (right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD226 Recombinant antibody
Catalog Number
98002-1-PBS
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
