CD226 Polyclonal antibody

CD226 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17842-1-AP

Synonyms

DNAX accessory molecule 1, DNAM-1, DNAM1, DNAM 1, CD226 molecule

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Validation Data Gallery

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  WB analysis using 17842-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 17842-1-AP (CD226 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17842-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17842-1-AP (CD226 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17842-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17842-1-AP (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Human peripheral blood platelets, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17842-1-AP targets CD226 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD226 fusion protein Ag12206
• Full Name: CD226 molecule
• Calculated Molecular Weight: 336 aa, 39 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC074787
• Gene Symbol: CD226
• Gene ID (NCBI): 10666
• RRID: AB_2072623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

Publications

Species	Application	Title
human,mouse	WB	Adv Sci (Weinh); Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells; Authors - Yidong Chen