CD22 Recombinant antibody, PBS Only

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  WB analysis using 83303-1-RR (same clone as 83303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83303-1-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83303-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83303-1-RR against Human CD22 were performed. The affinity constant is 3.33 nM.

CD22 {ptg:Brand} Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 83303-1-PBS

Clone No.230550C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

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Product Information

83303-1-PBS targets CD22 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD22 molecule
• Calculated Molecular Weight: 95kd
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001771.4
• Gene Symbol: CD22
• Gene ID (NCBI): 933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20273
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.