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CD22 Recombinant antibody, PBS Only

CD22 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230550C11

Cat no : 83303-1-PBS

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD22 Recombinant antibody (83303-1-RR)

    WB analysis using 83303-1-RR (same clone as 83303-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83303-1-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Recombinant antibody (83303-1-RR)

    IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Recombinant antibody (83303-1-RR)

    IHC staining of human tonsillitis using 83303-1-RR (same clone as 83303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83303-1-RR (CD22 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83303-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83303-1-RR

    Affinity and Kinetic Characterization of 83303-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83303-1-RR against Human CD22 were performed. The affinity constant is 3.33 nM.

Product Information

The immunogen of 83303-1-PBS is CD22 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD22 molecule
Calculated Molecular Weight95kd
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001771.4
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.