FITC Plus Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Antibody

CD21 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

BU32

Cat no : FITC-65512

Synonyms

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65512

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD19 and 5 ul FITC Plus Anti-Human CD21 Mouse Recombinant Antibody (FITC-65512, Clone:BU32) or FITC Plus Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65512

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD21 Mouse Recombinant Antibody (FITC-65512, Clone:BU32) or FITC Plus Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65512

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD19 and 5 ul FITC Plus Anti-Human CD21 Mouse Recombinant Antibody (FITC-65512, Clone:BU32) (left) or 5ul FITC Plus Anti-Human CD21 Mouse Monoclonal Antibody (FITC-65198, Clone:BU32) (right). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65512 targets CD21 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: complement component (3d/Epstein Barr virus) receptor 2
• GenBank Accession Number: BC090937
• Gene Symbol: CR2
• Gene ID (NCBI): 1380
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.