Anti-Human CD21 (BU32) Mouse IgG2a Recombinant Antibody, PBS Only

CD21 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU32

Cat no : 65591-1-PBS

Synonyms

EBV receptor, CR2, Complement receptor type 2, Complement C3d receptor, C3DR

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Validation Data Gallery

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  FC experiment of human PBMCs using 65591-1-MR (same clone as 65591-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65591-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65591-1-MR (same clone as 65591-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65591-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65591-1-MR (same clone as 65591-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD21 Mouse IgG2a Recombinant Antibody (65591-1-MR, Clone: BU32) (left) or 0.25 ug Anti-Human CD21 (65198-1-Ig, Clone: BU32) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65591-1-PBS in a different storage buffer formulation.

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Product Information

65591-1-PBS targets CD21 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: complement component (3d/Epstein Barr virus) receptor 2
• Calculated Molecular Weight: 1092 aa, 119 kDa
• GenBank Accession Number: BC136394
• Gene Symbol: CD21
• Gene ID (NCBI): 1380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.