CD206 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD206 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6A10

Cat no : 60143-1-PBS

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, Mannose receptor, mannose receptor, C type 1, MMR, MRC1

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Validation Data Gallery

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  WB analysis of human placenta using 60143-1-Ig (same clone as 60143-1-PBS)

  human placenta tissue was subjected to SDS PAGE followed by western blot with 60143-1-Ig (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 60143-1-Ig (same clone as 60143-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 60143-1-Ig (CD206 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IP experiment of human placenta using 60143-1-Ig (same clone as 60143-1-PBS)

  IP result of anti-CD206 (IP:60143-1-Ig, 5ug; Detection:60143-1-Ig 1:300) with human placenta tissue lysate 1520ug. This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60143-1-Ig (same clone as 60143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60143-1-Ig (same clone as 60143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60143-1-Ig (same clone as 60143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60143-1-Ig (same clone as 60143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 60143-1-Ig (same clone as 60143-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD206 antibody (60143-1-Ig, Clone: 2A6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 60143-1-Ig (same clone as 60143-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD206 antibody (60143-1-Ig, Clone: 2A6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60143-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60143-1-PBS targets CD206 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: MRC1
• Gene ID (NCBI): 4360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.