CD200R1 Polyclonal antibody

CD200R1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30013-1-AP

Synonyms

CD200 receptor 1, CD200R, CD200R1, CRTR2, HCRTR2, MOX2R, OX2R

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Validation Data Gallery

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  WB analysis using 30013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30013-1-AP (CD200R1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, K-562 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30013-1-AP targets CD200R1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: CD200 receptor 1
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC069743
• Gene Symbol: CD200R1
• Gene ID (NCBI): 131450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell surface glycoprotein CD200 receptor 1 (CD200R1) is also named as CD200R, CRTR2, MOX2R and OX2R, and belongs to the CD200R family. CD200R1 is inhibitory receptor for the CD200/OX2 cell surface glycoprotein. CD200R1 is highly glycosylated, containing ten potential N-glycosylation sites (PMID: 15187158). CD200R1 limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. As an immune inhibitory molecule, CD200R1 binds to CD200, ultimately leading to inhibition of classical activation of macrophages and microglia (PMID: 24588829).