Mouse CD200 Recombinant antibody, PBS Only

CD200 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240391A8

Cat no : 83437-5-PBS

Synonyms

OX2, Mox2, CD200 antigen, 240391A8

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Validation Data Gallery

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  WB analysis using 83437-5-RR (same clone as 83437-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83437-5-RR (CD200 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83437-5-RR (same clone as 83437-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83437-5-RR (same clone as 83437-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83437-5-RR (same clone as 83437-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83437-5-RR (same clone as 83437-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83437-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83437-5-RR against Mouse CD200 were performed. The affinity constant is 28.8 nM.

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Product Information

The immunogen of 83437-5-PBS is CD200 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD200 antigen
• Calculated Molecular Weight: 31 kDa
• Gene Symbol: Cd200
• Gene ID (NCBI): 17470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).