CoraLite® Plus 647 Anti-Human CD20 (2H7)
CD20 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2b, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2H7
Cat no : CL647-65085
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-65085 targets CD20 in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Human tonsillar B cells |
Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
Calculated Molecular Weight | 297 aa, 33 kDa |
GenBank Accession Number | BC002807 |
Gene Symbol | CD20 |
Gene ID (NCBI) | 931 |
RRID | AB_2883671 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD20 antibody CL647-65085 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |