Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone:2H7)(left) or 0.25 ug Anti-Human CD20 (65085-1-Ig, Clone: 2H7)(right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. lymphocyte were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

CD20 Recombinant Antibody for FC

Cat No. 65575-1-PBS

Clone No.2H7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

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Product Information

65575-1-PBS targets CD20 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human tonsillar B cells
• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).