FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65565-1-PBS
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65565-1-PBS
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
65565-1-PBS targets CD2 in FC applications and shows reactivity with human samples.
Store at -80°C. Stable for one year after shipment.
Background Information
CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65565-1-PBS
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65565-1-PBS
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CD2 Recombinant antibody
Catalog Number
65565-1-PBS
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet