Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only

CD2 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS1/8

Cat no : 65565-1-PBS

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65565-1-PBS

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65565-1-PBS

  1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65565-1-PBS targets CD2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.