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CD1A Monoclonal antibody, PBS Only

CD1A Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H7B2

Cat no : 60173-3-PBS

Synonyms

CD1, CD1A, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen, R4, T6

Validation Data Gallery

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  • Western Blot (WB) analysis of pig thymus tissue using CD1A Monoclonal antibody (60173-3-Ig)

    WB analysis of pig thymus using 60173-3-Ig (same clone as 60173-3-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60173-3-Ig (CD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60173-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD1A Monoclonal antibody (60173-3-Ig)

    WB analysis of Jurkat using 60173-3-Ig (same clone as 60173-3-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60173-3-Ig (CD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60173-3-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60173-3-PBS targets CD1A in WB, Indirect ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD1A fusion protein Ag11304
Full Name CD1a molecule
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC031645
Gene Symbol CD1A
Gene ID (NCBI) 909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.