PE Anti-Mouse CD197 (4B12)

CD197 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

4B12

Cat no : PE-65158

Synonyms

Ccr7, CD197, Cdw197, Cmkbr7, EBI1, Ebi1h

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using PE-65158

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 2.50 ug PE Anti-Mouse CD197 (PE-65158, Clone: 4B12) (red) or 2.50 ug isotype control antibody (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65158 targets CD197 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CCR7 Transfected Cell Line
• Full Name: chemokine (C-C motif) receptor 7
• Gene Symbol: Ccr7
• Gene ID (NCBI): 12775
• RRID: AB_2883936
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.