CD19 Polyclonal antibody

CD19 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27914-1-AP

Synonyms

Differentiation antigen CD19, CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4

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Validation Data Gallery

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  WB analysis using 27914-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27914-1-AP (CD19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 27914-1-AP

  IP result of anti-CD19 (IP:27914-1-AP, 4ug; Detection:27914-1-AP 1:3000) with Raji cells lysate 1800 ug.

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  IF Staining of Raji using 27914-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (27914-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IP detected in: Raji cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27914-1-AP targets CD19 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD19 fusion protein Ag27404
• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).