CD177 Polyclonal antibody

CD177 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18264-1-AP

Synonyms

CD177, CD177 antigen, CD177 molecule, HNA 2a, HNA2A, NB1, NB1 glycoprotein, NB1 GP, PRV 1, PRV1

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Validation Data Gallery

• 

  WB analysis of mouse bone marrow using 18264-1-AP

  mouse bone marrow tissue was subjected to SDS PAGE followed by western blot with 18264-1-AP (CD177 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse bone marrow

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18264-1-AP targets CD177 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD177 fusion protein Ag13146
• Full Name: CD177 molecule
• Calculated Molecular Weight: 437 aa, 46 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC029167
• Gene Symbol: CD177
• Gene ID (NCBI): 57126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).