PE Anti-Human CD16 (3G8)

CD16 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

3G8

Cat no : PE-65090

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using PE-65090

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3, and 5 ul PE Anti-Human CD16 (3G8) (PE-65090, Clone:3G8) or PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65090

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD16 (3G8) (PE-65090, Clone:3G8) (red) or PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21) (blue). Cells were not fixed. Lymphocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

PE-65090 targets CD16 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human PMN cells
• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC017865
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• RRID: AB_2883884
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

Publications

Species	Application	Title
human	FC	J Bone Miner Res; Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis; Authors - Lin Tao