Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody, PBS Only

CD16 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

3G8

Cat no : 65612-1-PBS

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone:3G8) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 65612-1-MR (same clone as 65612-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16 Mouse IgG2a Recombinant Antibody (65612-1-MR, Clone: 3G8) (left) or 0.25 ug Anti-Human CD16 (65090-1-Ig, Clone: 3G8) (right), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (right). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65612-1-PBS in a different storage buffer formulation.

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Product Information

65612-1-PBS targets CD16 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC017865
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).