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CD155/PVR Recombinant antibody, PBS Only

CD155/PVR Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241405F5

Cat no : 84138-5-PBS

Synonyms

PVR, NECL-5, NECL5, Necl 5, HVED

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD155/PVR Recombinant antibody (84138-5-RR)

    WB analysis using 84138-5-RR (same clone as 84138-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84138-5-RR (CD155/PVR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84138-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD155/PVR Recombinant antibody (84138-5-RR)

    IHC staining of human colon cancer using 84138-5-RR (same clone as 84138-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84138-5-RR (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84138-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD155/PVR Recombinant antibody (84138-5-RR)

    IHC staining of human colon cancer using 84138-5-RR (same clone as 84138-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84138-5-RR (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84138-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84138-5-RR

    Affinity and Kinetic Characterization of 84138-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84138-5-RR against Human CD155/PVR were performed. The affinity constant is 2.95 nM.

Product Information

The immunogen of 84138-5-PBS is CD155/PVR Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name poliovirus receptor
Calculated Molecular Weight 45 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC015542
Gene Symbol CD155/PVR
Gene ID (NCBI) 5817
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.