Anti-Human CD40L/CD154 (24-31)

CD40L/CD154 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

24-31

Cat no : 65156-1-Ig

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

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Validation Data Gallery

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  FC experiment of human PBMCs using 65156-1-Ig

  1X10^6 PMA (50 ng/ml) and ionomycin (1 ug/ml)-stimulated human PBMCs (5 hours) were surface stained with 0.2 ug Anti-Human CD154 (65156-1-Ig, Clone: 24-31) and FITC anti-mouse IgG1 Antibody at dilution 1:200 (green), or stained with 0.2 ug isotype control and FITC anti-mouse IgG1 Antibody at dilution 1:200 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65156-1-Ig targets CD40L/CD154 in FC applications and shows reactivity with Human, Non-human primates samples.

• Tested Reactivity: Human, Non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: human CD154 fusion protein
• Full Name: CD40 ligand
• Calculated Molecular Weight: 261 aa, 29 kDa
• GenBank Accession Number: BC071754
• Gene Symbol: CD40 Ligand
• Gene ID (NCBI): 959
• RRID: AB_2918438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.