Anti-Mouse CD40L/CD154 (MR1)

CD40L/CD154 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

MR1

Cat no : 65129-1-Ig

Synonyms

CD154, CD40 ligand, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly 62, Ly62, MR1, T BAM, Tnfsf5, TRAP

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Validation Data Gallery

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  FC experiment of BALB/c mouse CD4+ T cells using 65129-1-Ig

  1x10^6 unstimulated or PMA and ionomycin stimulated (overnight) BALB/c mouse CD4+ T cells were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse CD154 (65129-1-Ig, Clone: MR1) and FITC anti-Armenian Hamster IgG at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: PMA and ionomycin stimulated mouse CD4+ T cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65129-1-Ig targets CD40L/CD154 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD40 ligand
• GenBank Accession Number: BC119225
• Gene Symbol: CD154
• Gene ID (NCBI): 21947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.