CD151 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD151 Monoclonal Antibody for WB, IP, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A8G8

Cat no : 66567-1-PBS

Synonyms

CD151, CD151 antigen, GP27, Membrane glycoprotein SFA 1, MER2, PETA 3, RAPH, SFA1, Tetraspanin 24, Tspan 24, TSPAN24

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Validation Data Gallery

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  WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

  IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:10000) with human placenta tissue lysate 800 ug. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 66567-1-Ig (same clone as 66567-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CD151 (66567-1-Ig, Clone:2A8G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66567-1-PBS targets CD151 in WB, IP, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD151 fusion protein Ag25985
• Full Name: CD151 molecule (Raph blood group)
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001374
• Gene Symbol: CD151
• Gene ID (NCBI): 977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.