CD151 Polyclonal antibody, PBS Only

CD151 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27816-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 27816-1-AP (same clone as 27816-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 27816-1-AP (same clone as 27816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 27816-1-AP (same clone as 27816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 27816-1-AP (same clone as 27816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 27816-1-AP (same clone as 27816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

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Product Information

27816-1-PBS targets CD151 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD151 fusion protein Ag25968
• Full Name: CD151 molecule (Raph blood group)
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001374
• Gene Symbol: CD151
• Gene ID (NCBI): 977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.