CoraLite® Plus 488-conjugated CD147 Monoclonal antibody

CD147 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G12B5

Cat no : CL488-66443

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66443

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66443 (CD147 antibody) at dilution of 1:50.

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  IF Staining of human tonsillitis using CL488-66443

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66443 (CD147 antibody) at dilution of 1:50.

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  FC experiment of Jurkat using CL488-66443

  1X10^6 Jurkat cells were stained with 0.20ug CD147 antibody (CL488-66443, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66443 targets CD147 in IF, FC (Intra) applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD147 fusion protein Ag2619
• Full Name: basigin (Ok blood group)
• Calculated Molecular Weight: 385 aa, 42 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC009040
• Gene Symbol: BSG
• Gene ID (NCBI): 682
• RRID: AB_2883337
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211).