CD147 Recombinant antibody

CD147 Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230046A9

Cat no : 82909-7-RR

Synonyms

5F7, Basigin, basigin (Ok blood group), BSG, CD147, Collagenase stimulatory factor, EMMPRIN, HAb18G, M6, OK, OK blood group antigen, TCSF

---

Validation Data Gallery

• 

  WB analysis using 82909-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82909-7-RR (CD147 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 82909-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD147 antibody (82909-7-RR, Clone: 230046A9 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

• 

  FC experiment of HeLa using 82909-7-RR

  1x10^6 HeLa cells were surface stained with 0.25 ug CD147 Recombinant antibody (82909-7-RR, Clone: 230046A9) (red) or 0.25 ug Isotype Control (blue), and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were not fixed.

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82909-7-RR is CD147 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: basigin (Ok blood group)
• Calculated Molecular Weight: 385 aa, 42 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC009040
• Gene Symbol: BSG
• Gene ID (NCBI): 682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).